Calcium Test:
1. Fill a clean test tube with 5 ml of water to be tested (to the line on the tube).
2. Holding the bottle vertically, add 10 drops of Calcium Test Solution #1, cap the test tube, and shake for 10 seconds.
3. Shake Calcium Test Solution #2 vigorously for 10 seconds.
4. Holding the bottle vertically. Add Calcium Test Solution #2, drop by drop, to the test tube until the solution changes from pink to the blue endpoint. Be sure to count the number of drops added. Cap and shake the test tube between drops.
5. As the endpoint is approached. The color will usually change from pink to purple before finally changing to blue. After the purple color forms. Only 1-2 more drops should be required to reach the blue endpoint.
Phosphate Test:
1. Fill a clean test tube with 5 ml of water to be tested (to the line on the tube)
2. Holding the bottle vertically, add 6 drops from Phosphate Test Solution #1. Cap the test tube and shake vigorously for 5 seconds.
3. Now, holding the bottle vertically, add 6 drops from Phosphate Test Solution #2. Note: Test Solution #2 is a very thick solution and may require increased pressure to release drops.
4. Cap and shake the test tube vigorously for 5 seconds.
5. Wait 3 minutes for color to develop.
6. Read the test results by comparing the color of the solution to the phosphate color card. The tube should be viewed in a well-lit area against the white area of the cord. The closest match indicates the ppm Imp/Lb of total phosphate (into) in the water sample. Rinse the test tube with clean water after use.
Carbonate Hardness:
1. Fill a clean test tube with 5 ml of water to be tested (to the line on the tube).
2. Holding the bottle vertically add KH Test Solution drop at a time. Be sure to count the number of drops being added.
3. Cap the test tube & invert several times after each drop.
4. The test is completed when the water in the test tube is After having been shaken, it turns from blue to yellow. If you have difficulty discerning the color after the first drop of test solution is added. Remove the cap from the test tube and, holding it over a white background, look down through the tube.
5. The KH value is determined by the number of drops of test solution that must be added to turn the water in the test tube bright yellow. See KH Conversion Chart at the end of instructions to determine KH level.
Nitrate Test:
1. Fill a clean test tube with 5 ml of water to be tested (to the line on the tube).
2. Add 10 drops from Nitrate Test Solution #1, holding the dropper bottle upside down in a completely vertical position to assure uniformity of drops.
3. Cap the test tube and invert several times to mix solutions.
4. Vigorously shake the Nitrate Test Solution #2 for at least 30 seconds. This step is extremely important to ensure accuracy of test results.
5. Now add 10 drops of Horn Nitrate Test Solution #2, holding the dropper bottle upside down in a completely vertical position to assure uniformity of drops.
6. Cap the test tube and shake vigorously for 1 minute. This step is extremely important to ensure accuracy of test results.
7. Wait 5 minutes for the color to develop. 8. Read the test results by comparing the color of the solution to the nitrate color chart. The tube should be viewed in a well- It's an area against the white area of the cord. The closest match indicates the ppm (mg/L) of nitrate in the water sample. Rinse the test tube with clean water after use.